What is assay development

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IHC Hall of Fame. Conference Calendar. Join Us! About Us Corporate Profile. Interference Will components in the assay sample interfere with the assay? Reproducibility Does the assay display low inter- and intra-assay variability? Does the assay have sufficient discriminating power? Figure 1. Figure 2. Measurement of assay performance. Measurement of matrix metalloproteinase 9-mediated collagen type III degradation fragment as a marker of skin fibrosis.

BMC Dermatol. Coupland RA. Evaluation of enzyme inhibitors in drug discovery. New York: Wiley-Interscience; Assay development; fundamentals and principles. Jozefczuk J, Adjaye J. Quantitative real-time PCR-based analysis of gene expression. Methods Enzymol. A molecular assembly phase transition and kinetic proofreading modulate Ras activation by SOS. Targeted peptide measurements in biology and medicine: best practices for mass spectrometry-based assay development using a fit-for-purpose approach.

Mol Cell Proteomics. Langer G. Handb Exp Pharmacol. Temporal changes guided by mesenchymal stem cells on a 3D microgel platform enhance angiogenesis in vivo at a low-cell dose. Acute activation of AMP-activated protein kinase prevents H2O2-induced premature senescence in primary human keratinocytes. Effect of exercise intensity on skeletal muscle AMPK signaling in humans. Myocardial carnitine palmitoyltransferase I expression and long-chain fatty acid oxidation in fetal and newborn lambs.

A DNMT3B alternatively spliced exon and encoded peptide are novel biomarkers of human pluripotent stem cells. Relative importance of malonyl CoA and carnitine in maturation of fatty acid oxidation in newborn rabbit heart.

For this reason and to streamline your project, we provide an integrated approach to produce the best possible assay tools for assay development and optimization. Our approach is to: Define the biological question the assay has to respond to Identify the best suited biological model and assay technology in order to align relevance and realization time Thoroughly validate the assay and provide guidance on how to best interpret the results To facilitate assay development and optimization we take advantage of our integrated environment where customized reagents and biological systems can be produced.

Some examples could be: The production and qualification of recombinant proteins The production of probes of tool compounds in the form of small molecules, peptides or even antibodies The generation of modified cell lines for reporter systems and for validation purposes The use of relevant cells such as primary cells or iPSC The use of orthogonal biophysical readouts to validate an assay outcome.

We have successfully developed and validated assays for a number of purposes, such as: Enzymatic assays kinases, phosphatases, ubiquitin related enzymes, acetyl-transferases, deacetylases, methyl transferases, deglycosylase, metabolic enzymes, etc. Binding assays both for membrane proteins and for the protein-protein interaction targeted drug discovery programs Orthogonal biophysics readouts SPR, MS, NMR Cellular assays : reported based protein stability, interaction and transcriptional readouts immunoassays, phenotypic assays imaging , cytometry, etc.

Pharmacodynamic readouts The possibility of a therapeutic intervention is based on the modulation of the biology of a given biological system. Share this. IRBM Homepage.

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